Hemoglobin Subunit beta ELISA Kit

Catalog No. ABIN6810489

Product Details Hemoglobin Subunit beta ELISA Kit

Target: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human HbA1c/HBB ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human HbA1c.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for Hemoglobin Subunit beta

Target: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Alternative Name: HBB (HBB Products)

Synonyms: HBB ELISA Kit, HBB1 ELISA Kit, HBBA ELISA Kit, HBBB ELISA Kit, CD113t-C ELISA Kit, beta-globin ELISA Kit, hbb ELISA Kit, hemoglobin, beta ELISA Kit, hemoglobin subunit beta ELISA Kit, hemoglobin subunit beta-like ELISA Kit, beta globin ELISA Kit, hemoglobin beta chain complex ELISA Kit, hemoglobin subunit beta-1 ELISA Kit, HBB ELISA Kit, HBB2 ELISA Kit, LOC100720944 ELISA Kit, LOC480784 ELISA Kit, Hbb ELISA Kit, LOC100136576 ELISA Kit, LOC101098159 ELISA Kit, LOC100976465 ELISA Kit, LOC100410611 ELISA Kit, LOC105468624 ELISA Kit

Target Type: Viral Protein

UniProt: P68871