S100A7A ELISA Kit

Catalog No. ABIN6730824

Product Details S100A7A ELISA Kit

Target: S100A7A (S100 Calcium Binding Protein A7A (S100A7A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse S100A7A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse S100A7A.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for S100A7A

Target: S100A7A (S100 Calcium Binding Protein A7A (S100A7A))

Alternative Name: S100A7A (S100A7A Products)

Synonyms: NICE-2 ELISA Kit, S100A15 ELISA Kit, S100A7L1 ELISA Kit, S100A7f ELISA Kit, AY465109 ELISA Kit, Gm1020 ELISA Kit, S100a15 ELISA Kit, S100a15a ELISA Kit, S100a17l1 ELISA Kit, S100 calcium binding protein A7A ELISA Kit, Protein S100-A15A ELISA Kit, S100A7A ELISA Kit, S100a7a ELISA Kit, S100A15A ELISA Kit

UniProt: Q6S5I3

Pathways: Activation of Innate immune Response, S100 Proteins