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PPM1A ELISA Kit
PPM1A
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details PPM1A ELISA Kit
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Target
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PPM1A
(Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))
Reactivity
All reactivities for PPM1A elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Mouse PPM1A ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Mouse PPM1A.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for PPM1A
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Target
See all PPM1A ELISA Kits
PPM1A
(Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))
Alternative Name
PPM1A (PPM1A Products )
Synonyms
PP2C-ALPHA ELISA Kit, PP2CA ELISA Kit, PP2Calpha ELISA Kit, pp2ca1 ELISA Kit, ppp1r13b ELISA Kit, si:ch211-253p18.1 ELISA Kit, wu:fd42g12 ELISA Kit, zgc:153820 ELISA Kit, 2310003C21Rik ELISA Kit, 2900017D14Rik ELISA Kit, AI427932 ELISA Kit, AU017636 ELISA Kit, MMPa-2 ELISA Kit, MPPa-1 ELISA Kit, Pp2c1 ELISA Kit, pp2c-alpha ELISA Kit, pp2ca ELISA Kit, pp2calpha ELISA Kit, pp2ca2 ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent 1A ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent, 1Ab ELISA Kit, protein phosphatase 1A, magnesium dependent, alpha isoform ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent, 1A ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent 1A L homeolog ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent, 1Aa ELISA Kit, PPM1A ELISA Kit, ppm1ab ELISA Kit, Ppm1a ELISA Kit, ppm1a.L ELISA Kit, ppm1aa ELISA Kit
UniProt
P49443
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