EPH Receptor B4 ELISA Kit

Catalog No. ABIN6730734

Product Details EPH Receptor B4 ELISA Kit

Target: EPH Receptor B4 (EPHB4)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse EphB4 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse EphB4.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for EPH Receptor B4

Target: EPH Receptor B4 (EPHB4)

Alternative Name: EphB4 (EPHB4 Products)

Synonyms: HTK ELISA Kit, MYK1 ELISA Kit, TYRO11 ELISA Kit, htk ELISA Kit, myk1 ELISA Kit, ephB4a ELISA Kit, tyro11 ELISA Kit, ephb4b ELISA Kit, MGC130997 ELISA Kit, AI042935 ELISA Kit, Htk ELISA Kit, MDK2 ELISA Kit, Myk1 ELISA Kit, Tyro11 ELISA Kit, cb646 ELISA Kit, ephB4 ELISA Kit, rtk5 ELISA Kit, EphB-rtk8 ELISA Kit, cb417 ELISA Kit, rtk8 ELISA Kit, EPH receptor B4 ELISA Kit, EPH receptor B4 L homeolog ELISA Kit, EPH receptor B4 S homeolog ELISA Kit, Eph receptor B4 ELISA Kit, eph receptor B4a ELISA Kit, eph receptor B4b ELISA Kit, EPHB4 ELISA Kit, ephb4.L ELISA Kit, ephb4.S ELISA Kit, ephb4 ELISA Kit, Ephb4 ELISA Kit, ephb4a ELISA Kit, ephb4b ELISA Kit

UniProt: P54761

Pathways: RTK Signaling