EPH Receptor B3 ELISA Kit

Catalog No. ABIN6730733

Product Details EPH Receptor B3 ELISA Kit

Target: EPH Receptor B3 (EPHB3)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse EphB3 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse EphB3.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for EPH Receptor B3

Target: EPH Receptor B3 (EPHB3)

Alternative Name: EphB3 (EPHB3 Products)

Synonyms: tck ELISA Kit, MGC83457 ELISA Kit, EPHB3 ELISA Kit, ETK2 ELISA Kit, HEK2 ELISA Kit, TYRO6 ELISA Kit, AW456895 ELISA Kit, Cek10 ELISA Kit, Etk2 ELISA Kit, MDK5 ELISA Kit, Sek4 ELISA Kit, Tyro6 ELISA Kit, CEK10 ELISA Kit, EK10 ELISA Kit, ek3 ELISA Kit, ephb3 ELISA Kit, fc62d03 ELISA Kit, rtk3 ELISA Kit, wu:fc62d03 ELISA Kit, zek3 ELISA Kit, EPH receptor B3 ELISA Kit, EPH receptor B3 L homeolog ELISA Kit, Eph receptor B3 ELISA Kit, eph receptor B3a ELISA Kit, Eph receptor tyrosine kinase ELISA Kit, EPHB3 ELISA Kit, ephb3.L ELISA Kit, ephb3 ELISA Kit, Ephb3 ELISA Kit, ephb3a ELISA Kit

UniProt: P54754

Pathways: RTK Signaling