EPH Receptor A5 ELISA Kit

Catalog No. ABIN6730731

Product Details EPH Receptor A5 ELISA Kit

Target: EPH Receptor A5 (EPHA5)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse EphA5 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse EphA5.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for EPH Receptor A5

Target: EPH Receptor A5 (EPHA5)

Alternative Name: EphA5 (EPHA5 Products)

Synonyms: CEK7 ELISA Kit, EHK-1 ELISA Kit, EHK1 ELISA Kit, EK7 ELISA Kit, HEK7 ELISA Kit, TYRO4 ELISA Kit, AI854630 ELISA Kit, AW125296 ELISA Kit, Cek7 ELISA Kit, Ehk1 ELISA Kit, Els1 ELISA Kit, Hek7 ELISA Kit, Rek7 ELISA Kit, bsk ELISA Kit, Els1. ELISA Kit, EPHA5 ELISA Kit, EPH receptor A5 ELISA Kit, Eph receptor A5 ELISA Kit, EPHA5 ELISA Kit, epha5 ELISA Kit, Epha5 ELISA Kit

UniProt: Q60629

Pathways: RTK Signaling