PFKM ELISA Kit

Catalog No. ABIN6730720

Product Details PFKM ELISA Kit

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Muscle Phosphofructokinase/PFKM ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Muscle Phosphofructokinase.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: Muscle Phosphofructokinase (PFKM (PFKM Products)

Synonyms: GSD7 ELISA Kit, PFK-1 ELISA Kit, PFK1 ELISA Kit, PFKA ELISA Kit, PFKX ELISA Kit, AI131669 ELISA Kit, PFK-A ELISA Kit, PFK-M ELISA Kit, Pfk-4 ELISA Kit, Pfk4 ELISA Kit, Pfka ELISA Kit, Pfkx ELISA Kit, Pfk-M ELISA Kit, M-PFK ELISA Kit, PFKM ELISA Kit, pfkm ELISA Kit, zgc:92344 ELISA Kit, pfk ELISA Kit, im:7137110 ELISA Kit, wu:fc58g11 ELISA Kit, phosphofructokinase, muscle ELISA Kit, phosphofructokinase, platelet ELISA Kit, phosphofructokinase, muscle a ELISA Kit, phosphofructokinase, muscle S homeolog ELISA Kit, phosphofructokinase, muscle b ELISA Kit, PFKM ELISA Kit, Pfkm ELISA Kit, PFKP ELISA Kit, pfkma ELISA Kit, pfkm.S ELISA Kit, pfkmb ELISA Kit

UniProt: P08237

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect