Cholesterol Esterase ELISA Kit

Catalog No. ABIN6730707

Product Details Cholesterol Esterase ELISA Kit

Target: Cholesterol Esterase (CEL)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse CEL ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse CEL.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Cholesterol Esterase

Target: Cholesterol Esterase (CEL)

Alternative Name: CEL (CEL Products)

Synonyms: SC8F4.24 ELISA Kit, BAL ELISA Kit, BSDL ELISA Kit, BSSL ELISA Kit, CELL ELISA Kit, CEase ELISA Kit, FAP ELISA Kit, FAPP ELISA Kit, LIPA ELISA Kit, MODY8 ELISA Kit, 1810036E18Rik ELISA Kit, Bal ELISA Kit, Bssl ELISA Kit, carboxyl ester lipase ELISA Kit, cholesterol esterase ELISA Kit, hypothetical protein ELISA Kit, CEL ELISA Kit, SCO5420 ELISA Kit, Tfu_2331 ELISA Kit, SARE_RS01070 ELISA Kit, RSal33209_1252 ELISA Kit, Caci_5976 ELISA Kit, Ndas_0054 ELISA Kit, Micau_5972 ELISA Kit, ML5_2523 ELISA Kit, Cel ELISA Kit, cel ELISA Kit

UniProt: Q64285

Pathways: Lipid Metabolism