HSC70 Interacting Protein HIP ELISA Kit

Catalog No. ABIN6730680

Product Details HSC70 Interacting Protein HIP ELISA Kit

Target: HSC70 Interacting Protein HIP (ST13) (Suppression of Tumorigenicity 13 (ST13))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse Hip ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse Hip.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for HSC70 Interacting Protein HIP

Target: HSC70 Interacting Protein HIP (ST13) (Suppression of Tumorigenicity 13 (ST13))

Alternative Name: Hip (ST13 Products)

Synonyms: CG32789 ELISA Kit, Dmel\\CG32789 ELISA Kit, anon-EST:fe1A9 ELISA Kit, 1110007I03Rik ELISA Kit, 3110002K08Rik ELISA Kit, AW555194 ELISA Kit, HIP ELISA Kit, HOP ELISA Kit, HSPABP ELISA Kit, HSPABP1 ELISA Kit, PRO0786 ELISA Kit, SNC6 ELISA Kit, p48 ELISA Kit, AAG2 ELISA Kit, FAM10A1 ELISA Kit, FAM10A4 ELISA Kit, P48 ELISA Kit, Hsc/Hsp70-interacting protein ELISA Kit, suppression of tumorigenicity 13 ELISA Kit, ST13, Hsp70 interacting protein ELISA Kit, suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) ELISA Kit, HIP ELISA Kit, St13 ELISA Kit, ST13 ELISA Kit

UniProt: Q7TN16