EPH Receptor A2 ELISA Kit

Catalog No. ABIN6730664

Product Details EPH Receptor A2 ELISA Kit

Target: EPH Receptor A2 (EPHA2)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse EphA2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse EphA2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Alternative Name: EphA2 (EPHA2 Products)

Synonyms: ARCC2 ELISA Kit, CTPA ELISA Kit, CTPP1 ELISA Kit, CTRCT6 ELISA Kit, ECK ELISA Kit, epha2 ELISA Kit, MGC89505 ELISA Kit, AW545284 ELISA Kit, Eck ELISA Kit, Myk2 ELISA Kit, Sek-2 ELISA Kit, Sek2 ELISA Kit, id:ibd5152 ELISA Kit, rtk6 ELISA Kit, G42 ELISA Kit, XE10 ELISA Kit, epha2-A ELISA Kit, EPH receptor A2 ELISA Kit, Eph receptor A2 ELISA Kit, epoxide hydrolase ELISA Kit, eph receptor A2 a ELISA Kit, EPH receptor A2 L homeolog ELISA Kit, EPHA2 ELISA Kit, Epha2 ELISA Kit, epha2 ELISA Kit, ephA2 ELISA Kit, epha2a ELISA Kit, epha2.L ELISA Kit

UniProt: Q03145

Pathways: RTK Signaling