CPA1 ELISA Kit

Catalog No. ABIN6730601

Product Details CPA1 ELISA Kit

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse Carboxypeptidase A1/CPA1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse Carboxypeptidase A1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CPA1

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Alternative Name: Carboxypeptidase A1 (CPA1 (CPA1 Products)

Synonyms: cpa1 ELISA Kit, cbpa1 ELISA Kit, CPA ELISA Kit, CPA1 ELISA Kit, 0910001L12Rik ELISA Kit, Cpa ELISA Kit, carboxypeptidase A1 ELISA Kit, pancreatic carboxypeptidase A1 ELISA Kit, Carboxypeptidase A1 ELISA Kit, carboxypeptidase A1-like ELISA Kit, carboxypeptidase a1 ELISA Kit, carboxypeptidase A1, pancreatic ELISA Kit, CpipJ_CPIJ007169 ELISA Kit, CpipJ_CPIJ010802 ELISA Kit, CpipJ_CPIJ010805 ELISA Kit, MCYG_06789 ELISA Kit, MGYG_03840 ELISA Kit, cpa1 ELISA Kit, cbpa1 ELISA Kit, LOC100224866 ELISA Kit, CPA1 ELISA Kit, Cpa1 ELISA Kit

UniProt: Q7TPZ8