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CEACAM1 ELISA Kit
CEACAM1
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details CEACAM1 ELISA Kit
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Target
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CEACAM1
(Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))
Reactivity
All reactivities for CEACAM1 elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Mouse CEACAM-1/CD66a ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Mouse CEACAM-1.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for CEACAM1
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Target
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CEACAM1
(Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))
Alternative Name
CEACAM-1 (CD66a (CEACAM1 Products )
Synonyms
BGP ELISA Kit, BGP1 ELISA Kit, BGPI ELISA Kit, CD66e ELISA Kit, CEA ELISA Kit, zeh0068 ELISA Kit, hm:zeh0068 ELISA Kit, CEACAM8 ELISA Kit, CEACAM1 ELISA Kit, MGC142545 ELISA Kit, BGPR ELISA Kit, Bgp ELISA Kit, CD66a ELISA Kit, Ccam1 ELISA Kit, Bgp1 ELISA Kit, C-CAM ELISA Kit, Cc1 ELISA Kit, Cea-1 ELISA Kit, Cea-7 ELISA Kit, Cea1 ELISA Kit, Cea7 ELISA Kit, Hv-2 ELISA Kit, Hv2 ELISA Kit, MHVR ELISA Kit, MHVR1 ELISA Kit, Mhv-1 ELISA Kit, bb-1 ELISA Kit, mCEA1 ELISA Kit, mmCGM1 ELISA Kit, mmCGM1a ELISA Kit, mmCGM2 ELISA Kit, carcinoembryonic antigen related cell adhesion molecule 1 ELISA Kit, carcinoembryonic antigen related cell adhesion molecule 5 ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 1 ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 6 ELISA Kit, CEACAM1 ELISA Kit, CEACAM5 ELISA Kit, ceacam1 ELISA Kit, Ceacam1 ELISA Kit, LOC101103343 ELISA Kit
UniProt
P31809
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