ARL2BP ELISA Kit

Catalog No. ABIN6730576

Product Details ARL2BP ELISA Kit

Target: ARL2BP (ADP-Ribosylation Factor-Like 2 Binding Protein (ARL2BP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ARL2BP ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human ARL2BP.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for ARL2BP

Target: ARL2BP (ADP-Ribosylation Factor-Like 2 Binding Protein (ARL2BP))

Alternative Name: ARL2BP (ARL2BP Products)

Synonyms: arl2bp ELISA Kit, MGC81575 ELISA Kit, MGC84796 ELISA Kit, MGC130948 ELISA Kit, MGC107872 ELISA Kit, ARL2BP ELISA Kit, BART ELISA Kit, BART1 ELISA Kit, 1700010P10Rik ELISA Kit, 1700027H16Rik ELISA Kit, 6330544B05Rik ELISA Kit, AI482273 ELISA Kit, AI849834 ELISA Kit, Bart1 ELISA Kit, zgc:66308 ELISA Kit, zgc:77496 ELISA Kit, ADP ribosylation factor like GTPase 2 binding protein L homeolog ELISA Kit, ADP ribosylation factor like GTPase 2 binding protein ELISA Kit, ADP-ribosylation factor-like 2 binding protein ELISA Kit, ADP-ribosylation factor like GTPase 2 binding protein ELISA Kit, arl2bp.L ELISA Kit, arl2bp ELISA Kit, ARL2BP ELISA Kit, Arl2bp ELISA Kit

UniProt: Q9Y2Y0

Pathways: Maintenance of Protein Location