LSD1 ELISA Kit

Catalog No. ABIN6730574

Product Details LSD1 ELISA Kit

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Binding Specificity: pSer112

Reactivity: Human, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human and Mouse Phospho-LSD1 (S112) ELISA Kit. This assay semi-quantitatively measures phosphorylated LSD1 (Ser112) in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human and mouse LSD1 phosphorylated at Serine-112.

Characteristics:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for LSD1

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Alternative Name: LSD1 (KDM1A Products)

Synonyms: AOF2 ELISA Kit, BHC110 ELISA Kit, KDM1 ELISA Kit, LSD1 ELISA Kit, 1810043O07Rik ELISA Kit, AA408884 ELISA Kit, Aof2 ELISA Kit, D4Ertd478e ELISA Kit, Kdm1 ELISA Kit, Lsd1 ELISA Kit, mKIAA0601 ELISA Kit, DKFZp459N2450 ELISA Kit, RGD1562975 ELISA Kit, aof2 ELISA Kit, kdm1 ELISA Kit, wu:fb82e06 ELISA Kit, wu:fc51a02 ELISA Kit, wu:fj36a05 ELISA Kit, wu:fj66b06 ELISA Kit, KDM1A ELISA Kit, lsd1 ELISA Kit, xLSD1 ELISA Kit, AtLSD1 ELISA Kit, F9F13.30 ELISA Kit, F9F13_30 ELISA Kit, LESION SIMULATING DISEASE ELISA Kit, LESION SIMULATING DISEASE 1 ELISA Kit, lysine demethylase 1A ELISA Kit, lysine (K)-specific demethylase 1A ELISA Kit, lysine (K)-specific demethylase 1a ELISA Kit, LSD1 zinc finger family protein ELISA Kit, KDM1A ELISA Kit, Kdm1a ELISA Kit, kdm1a ELISA Kit, LSD1 ELISA Kit

Gene ID: 23028

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effect