CTSC ELISA Kit

Catalog No. ABIN6730547

Product Details CTSC ELISA Kit

Target: CTSC (Cathepsin C (CTSC))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse Cathepsin C/DPPI ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse Cathepsin C.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CTSC

Target: CTSC (Cathepsin C (CTSC))

Alternative Name: Cathepsin C (DPPI (CTSC Products)

Synonyms: CPPI ELISA Kit, DPP-I ELISA Kit, DPP1 ELISA Kit, DPPI ELISA Kit, HMS ELISA Kit, JP ELISA Kit, JPD ELISA Kit, PALS ELISA Kit, PDON1 ELISA Kit, PLS ELISA Kit, MGC69126 ELISA Kit, MGC107782 ELISA Kit, AI047818 ELISA Kit, CATC ELISA Kit, cb912 ELISA Kit, ik:tdsubc_1h2 ELISA Kit, sb:cb146 ELISA Kit, wu:fb34g12 ELISA Kit, wu:fj58d01 ELISA Kit, cathepsin C ELISA Kit, cathepsin C L homeolog ELISA Kit, cathepsin CPC1 ELISA Kit, CTSC ELISA Kit, ctsc.L ELISA Kit, ctsc ELISA Kit, TP03_0357 ELISA Kit, CMU_003360 ELISA Kit, CPC1 ELISA Kit, NAEGRDRAFT_80477 ELISA Kit, NAEGRDRAFT_37577 ELISA Kit, Ctsc ELISA Kit, Smp_183330 ELISA Kit

UniProt: P97821