×
For best experience we recommend to activate Javascript in your browser.
PRKAR1A ELISA Kit
PRKAR1A
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details PRKAR1A ELISA Kit
(hide)
Target
See all PRKAR1A products
PRKAR1A
(Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))
Reactivity
All reactivities for PRKAR1A elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Human PRKAR1A ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Human PRKAR1A.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
(show)
Application Details
(hide)
Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
(hide)
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for PRKAR1A
(hide)
Target
See all PRKAR1A products
PRKAR1A
(Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))
Alternative Name
PRKAR1A (PRKAR1A Products )
Synonyms
ACRDYS1 ELISA Kit, ADOHR ELISA Kit, CAR ELISA Kit, CNC ELISA Kit, CNC1 ELISA Kit, PKR1 ELISA Kit, PPNAD1 ELISA Kit, PRKAR1 ELISA Kit, TSE1 ELISA Kit, 1300018C22Rik ELISA Kit, RIalpha ELISA Kit, Tse-1 ELISA Kit, Tse1 ELISA Kit, RIIA ELISA Kit, prkar1a ELISA Kit, zgc:92515 ELISA Kit, im:7047729 ELISA Kit, cnc1 ELISA Kit, pkr1 ELISA Kit, ppnad1 ELISA Kit, prkar1 ELISA Kit, tse1 ELISA Kit, protein kinase cAMP-dependent type I regulatory subunit alpha ELISA Kit, protein kinase, cAMP dependent regulatory, type I, alpha ELISA Kit, protein kinase cAMP-dependent type 1 regulatory subunit alpha ELISA Kit, protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a ELISA Kit, cAMP-dependent protein kinase type I-alpha regulatory subunit pseudogene ELISA Kit, protein kinase cAMP-dependent type I regulatory subunit alpha S homeolog ELISA Kit, PRKAR1A ELISA Kit, Prkar1a ELISA Kit, prkar1aa ELISA Kit, LOC707275 ELISA Kit, prkar1a.S ELISA Kit
UniProt
P10644
Pathways
Hedgehog Signaling , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Myometrial Relaxation and Contraction , G-protein mediated Events , Interaction of EGFR with phospholipase C-gamma
Recently viewed
(hide)