DPP7 ELISA Kit

Catalog No. ABIN6730538

Product Details DPP7 ELISA Kit

Target: DPP7 (Dipeptidyl-Peptidase 7 (DPP7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse DPP7 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse DPP7.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for DPP7

Target: DPP7 (Dipeptidyl-Peptidase 7 (DPP7))

Alternative Name: DPP7 (DPP7 Products)

Synonyms: DPP2 ELISA Kit, DPPII ELISA Kit, QPP ELISA Kit, Dpp2 ELISA Kit, wu:fb19g02 ELISA Kit, zgc:112274 ELISA Kit, zgc:113564 ELISA Kit, DPP7 ELISA Kit, MGC151556 ELISA Kit, dipeptidyl peptidase 7 ELISA Kit, dipeptidylpeptidase 7 ELISA Kit, dipeptidyl-peptidase 7 ELISA Kit, DPP7 ELISA Kit, Dpp7 ELISA Kit, Shewana3_0712 ELISA Kit, Kkor_1052 ELISA Kit, Riean_0388 ELISA Kit, dpp7 ELISA Kit, Isop_1372 ELISA Kit

UniProt: Q9ET22