TPT1 ELISA Kit

Catalog No. ABIN6730534

Product Details TPT1 ELISA Kit

Target: TPT1 (Tumor Protein, Translationally-Controlled 1 (TPT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human TPT1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human TPT1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for TPT1

Target: TPT1 (Tumor Protein, Translationally-Controlled 1 (TPT1))

Alternative Name: TPT1 (TPT1 Products)

Synonyms: HRF ELISA Kit, TCTP ELISA Kit, p02 ELISA Kit, Trt ELISA Kit, p21 ELISA Kit, p23 ELISA Kit, TPT1 ELISA Kit, TCT1 ELISA Kit, tctp ELISA Kit, fa28a02 ELISA Kit, id:ibd2078 ELISA Kit, wu:fa28a02 ELISA Kit, PCHK23 ELISA Kit, hrf ELISA Kit, MGC79720 ELISA Kit, Tpt1 ELISA Kit, translationally controlled tumor protein ELISA Kit, CG4800 ELISA Kit, Dmel\\CG4800 ELISA Kit, dTCTP ELISA Kit, Tctp ELISA Kit, tumor protein, translationally-controlled 1 ELISA Kit, tumor protein, translationally-controlled 1 L homeolog ELISA Kit, translationally-controlled tumor protein ELISA Kit, translationally controlled tumor protein ELISA Kit, translationally controlled tumor-like protein ELISA Kit, Translationally controlled tumor protein ELISA Kit, translationally-controlled tumor protein homolog ELISA Kit, P23 tumor protein-like ELISA Kit, TPT1 ELISA Kit, Tpt1 ELISA Kit, tpt1 ELISA Kit, tpt1.L ELISA Kit, LOC711798 ELISA Kit, TCTP ELISA Kit, LOC548038 ELISA Kit, Tctp ELISA Kit, LOC108708523 ELISA Kit, LOC109404863 ELISA Kit, LOC105389397 ELISA Kit, LOC102577554 ELISA Kit

UniProt: P13693

Pathways: Response to Water Deprivation, Regulation of Cell Size, Negative Regulation of intrinsic apoptotic Signaling