UBE2H ELISA Kit

Catalog No. ABIN6730522

Product Details UBE2H ELISA Kit

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human UBE2H ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human UBE2H.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for UBE2H

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Alternative Name: UBE2H (UBE2H Products)

Synonyms: fc07a05 ELISA Kit, fi24c01 ELISA Kit, wu:fc07a05 ELISA Kit, wu:fi24c01 ELISA Kit, si:bz3c13.2 ELISA Kit, e2-20k ELISA Kit, ubc8 ELISA Kit, ubch ELISA Kit, ubch2 ELISA Kit, E2-20K ELISA Kit, GID3 ELISA Kit, UBC8 ELISA Kit, UBCH ELISA Kit, UBCH2 ELISA Kit, 1500009C23Rik ELISA Kit, AI181839 ELISA Kit, AI326965 ELISA Kit, AI462483 ELISA Kit, AW227540 ELISA Kit, Gid3 ELISA Kit, N28148 ELISA Kit, Ubc8 ELISA Kit, ubiquitin conjugating enzyme E2 H ELISA Kit, ubiquitin-conjugating enzyme E2 H ELISA Kit, Ubiquitin-conjugating enzyme E2 H ELISA Kit, ubiquitin conjugating enzyme E2H S homeolog ELISA Kit, ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) ELISA Kit, ubiquitin-conjugating enzyme E2-21 KD ELISA Kit, ubiquitin-conjugating enzyme E2H ELISA Kit, UBE2H ELISA Kit, CpipJ_CPIJ015105 ELISA Kit, Bm1_31760 ELISA Kit, PTRG_01621 ELISA Kit, LOAG_08716 ELISA Kit, ube2h.S ELISA Kit, ube2h ELISA Kit, uce-E2 ELISA Kit, uceE2 ELISA Kit, Ube2h ELISA Kit

UniProt: P62256