FGFR2 ELISA Kit

Catalog No. ABIN6730491

Product Details FGFR2 ELISA Kit

Target: FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse FGFR2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse FGFR2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for FGFR2

Target: FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))

Alternative Name: FGFR2 (FGFR2 Products)

Synonyms: FGFR2 ELISA Kit, FGFR2IIIb ELISA Kit, bek ELISA Kit, bfr-1 ELISA Kit, cd332 ELISA Kit, cek3 ELISA Kit, cfd1 ELISA Kit, ect1 ELISA Kit, fgfr-2 ELISA Kit, jws ELISA Kit, k-sam ELISA Kit, kgfr ELISA Kit, tk14 ELISA Kit, tk25 ELISA Kit, xfgfr2 ELISA Kit, DKFZp469H1521 ELISA Kit, BBDS ELISA Kit, BEK ELISA Kit, BFR-1 ELISA Kit, CD332 ELISA Kit, CEK3 ELISA Kit, CFD1 ELISA Kit, ECT1 ELISA Kit, JWS ELISA Kit, K-SAM ELISA Kit, KGFR ELISA Kit, TK14 ELISA Kit, TK25 ELISA Kit, AU043015 ELISA Kit, AW556123 ELISA Kit, Bek ELISA Kit, Fgfr-2 ELISA Kit, Fgfr-7 ELISA Kit, Fgfr7 ELISA Kit, KGFRTr ELISA Kit, svs ELISA Kit, FGF-R ELISA Kit, fgfr72IIIb ELISA Kit, FGFR ELISA Kit, fc56c05 ELISA Kit, wu:fc56c05 ELISA Kit, fibroblast growth factor receptor 2 ELISA Kit, fibroblast growth factor receptor 2 L homeolog ELISA Kit, FGFR2 ELISA Kit, LOC100084641 ELISA Kit, fgfr2 ELISA Kit, Fgfr2 ELISA Kit, fgfr2.L ELISA Kit

UniProt: P21803

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Growth Factor Binding