GAPDH ELISA Kit

Catalog No. ABIN6730487

Product Details GAPDH ELISA Kit

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse GAPDH ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse GAPDH.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH (GAPDH Products)

Synonyms: G3PD ELISA Kit, GAPD ELISA Kit, Gapd ELISA Kit, BEST:GH12586 ELISA Kit, CG12055 ELISA Kit, Dmel\\CG12055 ELISA Kit, GA3PDH ELISA Kit, GADPH ELISA Kit, GAP ELISA Kit, GAPDH ELISA Kit, GAPDH I ELISA Kit, GAPDH-1 ELISA Kit, GAPDH1 ELISA Kit, GAPDHI ELISA Kit, Gapdh ELISA Kit, Gapdh-1 ELISA Kit, Gapdh43E ELISA Kit, gadph ELISA Kit, gapdh ELISA Kit, gapdh-1 ELISA Kit, gh12586 ELISA Kit, cb609 ELISA Kit, gapd ELISA Kit, mg:bb02e05 ELISA Kit, wu:fb33a10 ELISA Kit, wu:ft80f05 ELISA Kit, KNC-NDS6 ELISA Kit, g3pd ELISA Kit, G3PDH ELISA Kit, glyceraldehyde-3-phosphate dehydrogenase ELISA Kit, Glyceraldehyde 3 phosphate dehydrogenase 1 ELISA Kit, glyceraldehyde-3-phosphate dehydrogenase S homeolog ELISA Kit, glyceraldehyde-3-phosphate dehydrogenase, type I ELISA Kit, olfactory receptor 8K3 ELISA Kit, GAPDH ELISA Kit, Gapdh ELISA Kit, Gapdh1 ELISA Kit, gapdh ELISA Kit, gapdh.S ELISA Kit, gapDH ELISA Kit, LOC100404960 ELISA Kit, LOC614985 ELISA Kit

UniProt: P16858