DCUN1D1 ELISA Kit

Catalog No. ABIN6730476

Product Details DCUN1D1 ELISA Kit

Target: DCUN1D1 (Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human DCUN1D1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human DCUN1D1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for DCUN1D1

Target: DCUN1D1 (Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1))

Alternative Name: DCUN1D1 (DCUN1D1 Products)

Synonyms: fd19a01 ELISA Kit, zgc:66414 ELISA Kit, wu:fd19a01 ELISA Kit, dcun1l1 ELISA Kit, rp42 ELISA Kit, sccro ELISA Kit, scro ELISA Kit, tes3 ELISA Kit, DCNL1 ELISA Kit, DCUN1L1 ELISA Kit, RP42 ELISA Kit, SCCRO ELISA Kit, SCRO ELISA Kit, Tes3 ELISA Kit, Rp42 ELISA Kit, pTes3 ELISA Kit, DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) ELISA Kit, DCN1-like protein 1 ELISA Kit, DCN1, defective in cullin neddylation 1, domain containing 1 L homeolog ELISA Kit, defective in cullin neddylation 1 domain containing 1 ELISA Kit, dcun1d1 ELISA Kit, dcnl1 ELISA Kit, dcun1d1.L ELISA Kit, DCUN1D1 ELISA Kit, Dcun1d1 ELISA Kit

UniProt: Q96GG9