CXCL14 ELISA Kit

Catalog No. ABIN6730469

Product Details CXCL14 ELISA Kit

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse CXCL14/BRAK ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse CXCL14.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CXCL14

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Alternative Name: CXCL14 (BRAK (CXCL14 Products)

Synonyms: BMAC ELISA Kit, BRAK ELISA Kit, KEC ELISA Kit, KS1 ELISA Kit, MIP-2g ELISA Kit, MIP2G ELISA Kit, NJAC ELISA Kit, SCYB14 ELISA Kit, 1110031L23Rik ELISA Kit, 1200006I23Rik ELISA Kit, AI414372 ELISA Kit, Kec ELISA Kit, MIP2gamma ELISA Kit, Scyb14 ELISA Kit, bolekine ELISA Kit, scyba ELISA Kit, fb67g04 ELISA Kit, wu:fb67g04 ELISA Kit, JSC ELISA Kit, bmac ELISA Kit, brak ELISA Kit, kec ELISA Kit, ks1 ELISA Kit, mip-2g ELISA Kit, mip2g ELISA Kit, njac ELISA Kit, scyb14 ELISA Kit, CXCL14 ELISA Kit, DKFZp469J175 ELISA Kit, C-X-C motif chemokine ligand 14 ELISA Kit, chemokine (C-X-C motif) ligand 14 ELISA Kit, chemokine (C-X-C motif) ligand 14 L homeolog ELISA Kit, CXCL14 ELISA Kit, Cxcl14 ELISA Kit, cxcl14 ELISA Kit, cxcl14.L ELISA Kit

UniProt: Q9WUQ5

Pathways: Autophagy