CSRP1 ELISA Kit

Catalog No. ABIN6730468

Product Details CSRP1 ELISA Kit

Target: CSRP1 (Cysteine and Glycine-Rich Protein 1 (CSRP1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse CSRP1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse CSRP1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CSRP1

Target: CSRP1 (Cysteine and Glycine-Rich Protein 1 (CSRP1))

Alternative Name: CSRP1 (CSRP1 Products)

Synonyms: CRP ELISA Kit, CRP1 ELISA Kit, CSRP ELISA Kit, CYRP ELISA Kit, D1S181E ELISA Kit, AA408841 ELISA Kit, AA959891 ELISA Kit, AW545626 ELISA Kit, Csrp ELISA Kit, cb796 ELISA Kit, csrp1 ELISA Kit, zgc:77332 ELISA Kit, MGC85577 ELISA Kit, MGC89932 ELISA Kit, CSRP1 ELISA Kit, zgc:158737 ELISA Kit, cysteine and glycine rich protein 1 ELISA Kit, cysteine and glycine-rich protein 1 ELISA Kit, cysteine and glycine-rich protein 1a ELISA Kit, cysteine and glycine rich protein 1 L homeolog ELISA Kit, cysteine and glycine-rich protein 1b ELISA Kit, Cysteine and glycine-rich protein 1 ELISA Kit, CSRP1 ELISA Kit, Csrp1 ELISA Kit, csrp1a ELISA Kit, csrp1.L ELISA Kit, csrp1 ELISA Kit, csrp1b ELISA Kit, LOC100304834 ELISA Kit, Tsp_01053 ELISA Kit

UniProt: P97315