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TDGF1 ELISA Kit
TDGF1
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details TDGF1 ELISA Kit
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Target
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TDGF1
(Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
Reactivity
All reactivities for TDGF1 elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Mouse Cripto ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Mouse Cripto.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for TDGF1
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Target
See all TDGF1 ELISA Kits
TDGF1
(Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
Alternative Name
Cripto (TDGF1 Products )
Synonyms
CR ELISA Kit, CRGF ELISA Kit, CRIPTO ELISA Kit, CR1 ELISA Kit, cripto ELISA Kit, cb85 ELISA Kit, tdgf1 ELISA Kit, wu:fe01d04 ELISA Kit, zgc:109829 ELISA Kit, Cripto-1 ELISA Kit, cr1 ELISA Kit, cr1a ELISA Kit, frl-1 ELISA Kit, frl1 ELISA Kit, xcr1 ELISA Kit, TDGF1 ELISA Kit, tdgf3 ELISA Kit, cripto-3 ELISA Kit, teratocarcinoma-derived growth factor 1 ELISA Kit, teratocarcinoma-derived growth factor 1, member 3 L homeolog ELISA Kit, teratocarcinoma-derived growth factor 1, member 1 ELISA Kit, TDGF1 ELISA Kit, Tdgf1 ELISA Kit, tdgf1 ELISA Kit, tdgf1.3.L ELISA Kit, tdgf1.1 ELISA Kit
UniProt
P51865
Pathways
EGFR Signaling Pathway
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