CPNE1 ELISA Kit

Catalog No. ABIN6730461

Product Details CPNE1 ELISA Kit

Target: CPNE1 (Copine I (CPNE1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Copine-1/CPNE1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Copine-1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CPNE1

Target: CPNE1 (Copine I (CPNE1))

Alternative Name: Copine-1 (CPNE1 (CPNE1 Products)

Synonyms: CPNE1 ELISA Kit, MGC108079 ELISA Kit, COPN1 ELISA Kit, CPN1 ELISA Kit, 1810028N16Rik ELISA Kit, mKIAA4108 ELISA Kit, cpne3 ELISA Kit, cpne3l ELISA Kit, wu:fb69d07 ELISA Kit, wu:fc45a04 ELISA Kit, zgc:55873 ELISA Kit, copine I ELISA Kit, copine 1 ELISA Kit, RNA-binding protein 12 ELISA Kit, copine-1 ELISA Kit, copine I L homeolog ELISA Kit, CPNE1 ELISA Kit, cpne1 ELISA Kit, CPNE1b ELISA Kit, LOC100032499 ELISA Kit, LOC100484845 ELISA Kit, EDI_033120 ELISA Kit, LOC100281056 ELISA Kit, cpne1.L ELISA Kit, Cpne1 ELISA Kit

UniProt: Q99829