FDPS ELISA Kit

Catalog No. ABIN6730379

Product Details FDPS ELISA Kit

Target: FDPS (Farnesyl Diphosphate Synthase (FDPS))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse FDPS ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse FDPS.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for FDPS

Target: FDPS (Farnesyl Diphosphate Synthase (FDPS))

Alternative Name: FDPS (FDPS Products)

Synonyms: CG12389 ELISA Kit, DmFPPS ELISA Kit, DmFPPSase ELISA Kit, DmFPS ELISA Kit, Dmel\\CG12389 ELISA Kit, FPS ELISA Kit, fpps ELISA Kit, fps ELISA Kit, l(2)k06103 ELISA Kit, v(2)k03514 ELISA Kit, NCU01175.1 ELISA Kit, 16.m00760 ELISA Kit, DDBDRAFT_0206220 ELISA Kit, DDBDRAFT_0215017 ELISA Kit, DDB_0206220 ELISA Kit, DDB_0215017 ELISA Kit, FPPS ELISA Kit, Ac2-125 ELISA Kit, (2E ELISA Kit, 6030492I17Rik ELISA Kit, AI256750 ELISA Kit, Fdpsl1 ELISA Kit, mKIAA1293 ELISA Kit, Farnesyl pyrophosphate synthase ELISA Kit, farnesyl diphosphate synthase ELISA Kit, farnesyl diphosphate synthase L homeolog ELISA Kit, farnesyl pyrophosphate synthase ELISA Kit, farnesyl pyrophosphate synthetase ELISA Kit, farnesyl-pyrophosphate synthetase ELISA Kit, FDP synthase ELISA Kit, Farnesyl pyrophosphate synthetase ELISA Kit, farnesyl diphosphate synthetase ELISA Kit, farnesyl pyrophosphate synthase 1 ELISA Kit, Fpps ELISA Kit, fdps ELISA Kit, fdps.L ELISA Kit, MGG_08649 ELISA Kit, TP03_0857 ELISA Kit, Tb09.160.4300 ELISA Kit, fpp ELISA Kit, BBOV_I000120 ELISA Kit, fps ELISA Kit, fpps ELISA Kit, FDPS ELISA Kit, Fdps ELISA Kit, fps1 ELISA Kit

UniProt: Q920E5

Pathways: Regulation of Muscle Cell Differentiation