MANF ELISA Kit

Catalog No. ABIN6730370

Product Details MANF ELISA Kit

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse MANF ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse MANF.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: MANF (MANF Products)

Synonyms: ARMET ELISA Kit, ARP ELISA Kit, 3230402M22Rik ELISA Kit, AA407711 ELISA Kit, AA408789 ELISA Kit, AA673178 ELISA Kit, Armet ELISA Kit, D17914 ELISA Kit, D18Mgi17 ELISA Kit, MANF ELISA Kit, RBM15B ELISA Kit, ARP-like ELISA Kit, CG7013 ELISA Kit, DmMANF ELISA Kit, Dmel\\CG7013 ELISA Kit, anon-EST:ParkEST188 ELISA Kit, arp ELISA Kit, armet ELISA Kit, im:6909481 ELISA Kit, zgc:153327 ELISA Kit, manf ELISA Kit, mesencephalic astrocyte derived neurotrophic factor ELISA Kit, mesencephalic astrocyte-derived neurotrophic factor ELISA Kit, RNA binding motif protein 15B ELISA Kit, Mesencephalic astrocyte-derived neurotrophic factor ELISA Kit, mesencephalic astrocyte-derived neurotrophic factor S homeolog ELISA Kit, MANF ELISA Kit, Manf ELISA Kit, RBM15B ELISA Kit, manf ELISA Kit, manf.S ELISA Kit

UniProt: Q9CXI5