PLA2G1B ELISA Kit

Catalog No. ABIN6730357

Product Details PLA2G1B ELISA Kit

Target: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse PLA2G1B ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse PLA2G1B.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PLA2G1B

Target: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Alternative Name: PLA2G1B (PLA2G1B Products)

Synonyms: C133 ELISA Kit, CG4346 ELISA Kit, Dmel\\CG42237 ELISA Kit, Dmel_CG4346 ELISA Kit, PLA2 ELISA Kit, GB13351 ELISA Kit, bvPLA2 ELISA Kit, PLA2A ELISA Kit, PPLA2 ELISA Kit, sPLA(2)-IB ELISA Kit, Pla2a ELISA Kit, sPLA2IB ELISA Kit, PLA2G1B ELISA Kit, CG42237 gene product from transcript CG42237-RB ELISA Kit, phospholipase A2 ELISA Kit, phospholipase A2 group IB ELISA Kit, phospholipase A2, group IB, pancreas ELISA Kit, phospholipase A2 group IVA ELISA Kit, CG42237 ELISA Kit, PLA2 ELISA Kit, LOC100631065 ELISA Kit, Pla2 ELISA Kit, PLA2G1B ELISA Kit, Pla2g1b ELISA Kit, PLA2G4A ELISA Kit

UniProt: Q9Z0Y2

Pathways: Inositol Metabolic Process, VEGF Signaling