IL1F9 ELISA Kit

Catalog No. ABIN6730341

Product Details IL1F9 ELISA Kit

Target: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse IL36G ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse IL36G.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for IL1F9

Target: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Alternative Name: IL36G (IL1F9 Products)

Synonyms: IL-1F9 ELISA Kit, IL-1H1 ELISA Kit, IL-1RP2 ELISA Kit, IL1E ELISA Kit, IL1F9 ELISA Kit, IL1H1 ELISA Kit, IL1RP2 ELISA Kit, Il36g ELISA Kit, Il1f9 ELISA Kit, RGD1563019 ELISA Kit, IL36G ELISA Kit, interleukin 36 gamma ELISA Kit, interleukin 1 family, member 9 ELISA Kit, interleukin 36, gamma ELISA Kit, interleukin-36 gamma ELISA Kit, IL36G ELISA Kit, Il1f9 ELISA Kit, Il36g ELISA Kit, LOC100065031 ELISA Kit

UniProt: Q8R460