BMPER ELISA Kit

Catalog No. ABIN6730328

Product Details BMPER ELISA Kit

Target: BMPER (BMP Binding Endothelial Regulator (BMPER))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse Crossveinless-2/CV-2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse Crossveinless-2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for BMPER

Target: BMPER (BMP Binding Endothelial Regulator (BMPER))

Alternative Name: Crossveinless-2 (CV-2 (BMPER Products)

Synonyms: BMPER ELISA Kit, CG15671 ELISA Kit, CT35855 ELISA Kit, Cv 2 ELISA Kit, Cv-2 ELISA Kit, Cv2 ELISA Kit, Dmel\\CG15671 ELISA Kit, cv 2 ELISA Kit, CV2 ELISA Kit, cv-2 ELISA Kit, crim3 ELISA Kit, cv2 ELISA Kit, cvl2 ELISA Kit, id:ibd5071 ELISA Kit, CV-2 ELISA Kit, CRIM3 ELISA Kit, 3110056H04Rik ELISA Kit, Crim3 ELISA Kit, RGD1563373 ELISA Kit, BMP binding endothelial regulator ELISA Kit, crossveinless 2 ELISA Kit, BMP-binding endothelial regulator protein ELISA Kit, BMP binding endothelial regulator L homeolog ELISA Kit, BMP-binding endothelial regulator ELISA Kit, BMPER ELISA Kit, cv-2 ELISA Kit, bmper ELISA Kit, LOC100070275 ELISA Kit, cv2 ELISA Kit, bmper.L ELISA Kit, Bmper ELISA Kit

UniProt: Q8CJ69