TWSG1 ELISA Kit

Catalog No. ABIN6730321

Product Details TWSG1 ELISA Kit

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse TSG ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse TSG.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for TWSG1

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Alternative Name: TSG (TWSG1 Products)

Synonyms: TWSG1 ELISA Kit, TSG ELISA Kit, DKFZp469J051 ELISA Kit, 1810013J15Rik ELISA Kit, 9030422N06Rik ELISA Kit, AW552143 ELISA Kit, D17Ertd403e ELISA Kit, Tsg ELISA Kit, Twg ELISA Kit, fi23f01 ELISA Kit, tsg ELISA Kit, tsga ELISA Kit, twsg1 ELISA Kit, wu:fi23f01 ELISA Kit, twsg ELISA Kit, tsg1 ELISA Kit, tsgb ELISA Kit, ztsg1 ELISA Kit, twisted gastrulation BMP signaling modulator 1 ELISA Kit, twisted gastrulation homolog 1 (Drosophila) ELISA Kit, twisted gastrulation BMP signaling modulator 1a ELISA Kit, twisted gastrulation BMP signaling modulator 1 S homeolog ELISA Kit, twisted gastrulation BMP signaling modulator 1 L homeolog ELISA Kit, twisted gastrulation BMP signaling modulator 1b ELISA Kit, TWSG1 ELISA Kit, Twsg1 ELISA Kit, twsg1a ELISA Kit, twsg1.S ELISA Kit, twsg1.L ELISA Kit, twsg1b ELISA Kit

UniProt: Q9EP52