Syndecan 1 ELISA Kit

Catalog No. ABIN6730295

Product Details Syndecan 1 ELISA Kit

Target: Syndecan 1 (SDC1)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse Syndecan-1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse Syndecan-1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Syndecan 1

Target: Syndecan 1 (SDC1)

Alternative Name: Syndecan-1 (SDC1 Products)

Synonyms: CG10497 ELISA Kit, CT27296 ELISA Kit, Dmel\\CG10497 ELISA Kit, Dsyn ELISA Kit, Syd ELISA Kit, Syn ELISA Kit, dSdc ELISA Kit, l(2)10608 ELISA Kit, sdc ELISA Kit, syd ELISA Kit, syn ELISA Kit, Syn1 ELISA Kit, cd138 ELISA Kit, synd1 ELISA Kit, xSyn1 ELISA Kit, syndecan1 ELISA Kit, Syndecan-1 ELISA Kit, XSyndecan-1 ELISA Kit, SDC1 ELISA Kit, CD138 ELISA Kit, SDC ELISA Kit, SYND1 ELISA Kit, syndecan ELISA Kit, AA408134 ELISA Kit, AA409076 ELISA Kit, Sstn ELISA Kit, Synd ELISA Kit, Synd1 ELISA Kit, syn-1 ELISA Kit, HSPG ELISA Kit, SYNDECA ELISA Kit, Syndecan ELISA Kit, Syndecan ELISA Kit, syndecan 1 ELISA Kit, Sdc ELISA Kit, sdc1 ELISA Kit, SDC1 ELISA Kit, Sdc1 ELISA Kit

UniProt: P18828

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism