RAB6A ELISA Kit

Catalog No. ABIN6730273

Product Details RAB6A ELISA Kit

Target: RAB6A (RAB6A, Member RAS Oncogene Family (RAB6A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human RAB6A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human RAB6A.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for RAB6A

Target: RAB6A (RAB6A, Member RAS Oncogene Family (RAB6A))

Alternative Name: RAB6A (RAB6A Products)

Synonyms: fb43c01 ELISA Kit, wu:fb43c01 ELISA Kit, zgc:55788 ELISA Kit, rab6 ELISA Kit, MGC53077 ELISA Kit, rab6b ELISA Kit, rab6a' ELISA Kit, MGC76176 ELISA Kit, LOC692966 ELISA Kit, RAB6 ELISA Kit, 2610028L11Rik ELISA Kit, AA419671 ELISA Kit, Rab6 ELISA Kit, RCO4-3 ELISA Kit, Rab6b ELISA Kit, RAB6A, member RAS oncogene family ELISA Kit, RAB6A, member RAS oncogene family S homeolog ELISA Kit, ras small monomeric GTPase Rab6 ELISA Kit, ras-related protein Rab-6A ELISA Kit, rab6a ELISA Kit, rab6a.S ELISA Kit, LOC692966 ELISA Kit, RAB6A ELISA Kit, Rab6a ELISA Kit

UniProt: P20340