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Olfactomedin 1 ELISA Kit

OLFM1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.084 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN6730244
  • Target See all Olfactomedin 1 (OLFM1) ELISA Kits
    Olfactomedin 1 (OLFM1)
    Reactivity
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.084 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.084 ng/mL
    Application
    ELISA
    Purpose
    Mouse Olfactomedin-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Mouse Olfactomedin-1.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Reagent Preparation

    Recommended Dilution for serum and plasma samples10 fold

    Restrictions
    For Research Use only
  • Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all Olfactomedin 1 (OLFM1) ELISA Kits
    Olfactomedin 1 (OLFM1)
    Alternative Name
    Olfactomedin-1 (OLFM1 Products)
    Synonyms
    MGC132187 ELISA Kit, OLFM1 ELISA Kit, amy ELISA Kit, noe1 ELISA Kit, olfa ELISA Kit, noelin1 ELISA Kit, noelin-1 ELISA Kit, AMY ELISA Kit, NOE1 ELISA Kit, NOELIN1 ELISA Kit, OlfA ELISA Kit, AW742568 ELISA Kit, Noe1 ELISA Kit, Pancortin ELISA Kit, Pancortin3 ELISA Kit, D2Sut1e ELISA Kit, NOELIN-1 ELISA Kit, olfactomedin 1 ELISA Kit, olfactomedin 1 S homeolog ELISA Kit, OLFM1 ELISA Kit, olfm1.S ELISA Kit, olfm1 ELISA Kit, Olfm1 ELISA Kit
    UniProt
    O88998
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