S100A8/A9 Complex (Calprotectin) ELISA Kit

Catalog No. ABIN6730234

Product Details S100A8/A9 Complex (Calprotectin) ELISA Kit

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 35 pg/mL - 8000 pg/mL

Minimum Detection Limit: 35 pg/mL

Application: ELISA

Purpose: Human Calprotectin L1/S100-A8/A9 Complex ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Calprotectin L1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Reagent Preparation:

Recommended Dilution for serum and plasma samples4000 fold

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))

Alternative Name: Calprotectin L1 (S100-A8/A9 Complex) (S100A8/A9 Products)

Synonyms: 60B8AG ELISA Kit, CAGB ELISA Kit, CFAG ELISA Kit, CGLB ELISA Kit, L1AG ELISA Kit, LIAG ELISA Kit, MAC387 ELISA Kit, MIF ELISA Kit, MRP14 ELISA Kit, NIF ELISA Kit, P14 ELISA Kit, S100 calcium binding protein A9 ELISA Kit, S100A9 ELISA Kit

Pathways: S100 Proteins