CD44 ELISA Kit

Catalog No. ABIN6730117

Product Details CD44 ELISA Kit

Target: CD44

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse CD44 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse CD44.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CD44

Target: CD44

Alternative Name: CD44 (CD44 Products)

Synonyms: CDW44 ELISA Kit, CSPG8 ELISA Kit, ECMR-III ELISA Kit, HCELL ELISA Kit, HUTCH-I ELISA Kit, IN ELISA Kit, LHR ELISA Kit, MC56 ELISA Kit, MDU2 ELISA Kit, MDU3 ELISA Kit, MIC4 ELISA Kit, Pgp1 ELISA Kit, AU023126 ELISA Kit, AW121933 ELISA Kit, AW146109 ELISA Kit, HERMES ELISA Kit, Ly-24 ELISA Kit, Pgp-1 ELISA Kit, CD44A ELISA Kit, METAA ELISA Kit, RHAMM ELISA Kit, XCD44 ELISA Kit, cd44 ELISA Kit, cdw44 ELISA Kit, cspg8 ELISA Kit, ecmr-iii ELISA Kit, hcell ELISA Kit, lhr ELISA Kit, mc56 ELISA Kit, mdu2 ELISA Kit, mdu3 ELISA Kit, mic4 ELISA Kit, mutch-i ELISA Kit, pgp1 ELISA Kit, CD44 molecule (Indian blood group) ELISA Kit, syndecan 4 ELISA Kit, CD44 antigen ELISA Kit, CD44 molecule (Indian blood group) S homeolog ELISA Kit, CD44 ELISA Kit, SDC4 ELISA Kit, Cd44 ELISA Kit, cd44.S ELISA Kit

UniProt: P15379

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling