EpCAM ELISA Kit

Catalog No. ABIN6730115

Product Details EpCAM ELISA Kit

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse EpCAM/TROP-1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse EpCAM.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM (TROP-1 (EPCAM Products)

Synonyms: DIAR5 ELISA Kit, EGP-2 ELISA Kit, EGP314 ELISA Kit, EGP40 ELISA Kit, ESA ELISA Kit, HNPCC8 ELISA Kit, KS1/4 ELISA Kit, KSA ELISA Kit, M4S1 ELISA Kit, MIC18 ELISA Kit, MK-1 ELISA Kit, TACSTD1 ELISA Kit, TROP1 ELISA Kit, ECS-1 ELISA Kit, ECS1 ELISA Kit, ESA1 ELISA Kit, M17S1 ELISA Kit, EGP ELISA Kit, Ep-CAM ELISA Kit, sb:cb6 ELISA Kit, tacstd ELISA Kit, wu:fj17g02 ELISA Kit, zgc:110304 ELISA Kit, zgc:77119 ELISA Kit, tacstd1 ELISA Kit, MGC80540 ELISA Kit, EPCAM ELISA Kit, egp ELISA Kit, ksa ELISA Kit, m4s1 ELISA Kit, mk-1 ELISA Kit, cd326 ELISA Kit, egp40 ELISA Kit, mic18 ELISA Kit, trop1 ELISA Kit, ep-cam ELISA Kit, hegp-2 ELISA Kit, co17-1a ELISA Kit, ga733-2 ELISA Kit, CD326 ELISA Kit, Egp314 ELISA Kit, EpCAM1 ELISA Kit, GA733-2 ELISA Kit, Ly74 ELISA Kit, Tacsd1 ELISA Kit, Tacstd1 ELISA Kit, gp40 ELISA Kit, epithelial cell adhesion molecule ELISA Kit, flotillin 2 ELISA Kit, epithelial cell adhesion molecule S homeolog ELISA Kit, EPCAM ELISA Kit, FLOT2 ELISA Kit, epcam ELISA Kit, epcam.S ELISA Kit, Epcam ELISA Kit

UniProt: Q99JW5