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TNFRSF11A ELISA Kit
TNFRSF11A
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details TNFRSF11A ELISA Kit
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Target
See all TNFRSF11A ELISA Kits
TNFRSF11A
(Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
Reactivity
All reactivities for TNFRSF11A elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Mouse RANK/TNFRSF11A ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Mouse RANK.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for TNFRSF11A
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Target
See all TNFRSF11A ELISA Kits
TNFRSF11A
(Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
Alternative Name
RANK (TNFRSF11A (TNFRSF11A Products )
Synonyms
RANK ELISA Kit, RGD1563614 ELISA Kit, TNFRSF11A ELISA Kit, Ly109 ELISA Kit, ODFR ELISA Kit, OFE ELISA Kit, Rank ELISA Kit, TRANCE-R ELISA Kit, mRANK ELISA Kit, CD265 ELISA Kit, FEO ELISA Kit, LOH18CR1 ELISA Kit, OPTB7 ELISA Kit, OSTS ELISA Kit, PDB2 ELISA Kit, TRANCER ELISA Kit, TNF receptor superfamily member 11A ELISA Kit, TNF receptor superfamily member 11a ELISA Kit, tumor necrosis factor receptor superfamily, member 11a, NFKB activator ELISA Kit, Tnfrsf11a ELISA Kit, TNFRSF11A ELISA Kit
UniProt
O35305
Pathways
NF-kappaB Signaling
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