Integrin beta 3 ELISA Kit

Catalog No. ABIN6730101

Product Details Integrin beta 3 ELISA Kit

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Integrin beta 3/CD61 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Integrin beta 3.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: Integrin beta 3 (CD61 (ITGB3 Products)

Synonyms: BDPLT16 ELISA Kit, BDPLT2 ELISA Kit, CD61 ELISA Kit, GP3A ELISA Kit, GPIIIa ELISA Kit, GT ELISA Kit, INGRB3 ELISA Kit, integrin ELISA Kit, INTB3 ELISA Kit, GPIIIA ELISA Kit, cd61 ELISA Kit, gp3a ELISA Kit, itgb3 ELISA Kit, itgb3.2 ELISA Kit, itgb3.1 ELISA Kit, integrin subunit beta 3 ELISA Kit, integrin beta 3 ELISA Kit, integrin subunit beta 3 L homeolog ELISA Kit, integrin beta 3a ELISA Kit, integrin beta 3b ELISA Kit, ITGB3 ELISA Kit, Itgb3 ELISA Kit, itgb3.L ELISA Kit, itgb3a ELISA Kit, itgb3b ELISA Kit

UniProt: Q9Y6R9

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex