PGD ELISA Kit

Catalog No. ABIN6730055

Product Details PGD ELISA Kit

Target: PGD (Phosphogluconate Dehydrogenase (PGD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human PGD ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human PGD.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PGD

Target: PGD (Phosphogluconate Dehydrogenase (PGD))

Alternative Name: PGD (PGD Products)

Synonyms: 6PGD ELISA Kit, 6-PGD ELISA Kit, 6-Pgd ELISA Kit, 6-pgd ELISA Kit, 6PGDH ELISA Kit, 6Pgd ELISA Kit, 6Pgdh ELISA Kit, 6pgdh ELISA Kit, A7 ELISA Kit, CG3724 ELISA Kit, Dmel\\CG3724 ELISA Kit, EG:87B1.4 ELISA Kit, N1 ELISA Kit, PGD ELISA Kit, Pdg ELISA Kit, l(1)2Dc ELISA Kit, l(1)2De ELISA Kit, l(1)A7 ELISA Kit, l(1)G0385 ELISA Kit, l(1)N1 ELISA Kit, l(1)N3[90] ELISA Kit, l(1)Pgd ELISA Kit, l(1)Pgd-A ELISA Kit, l35 ELISA Kit, pgd ELISA Kit, 0610042A05Rik ELISA Kit, AU019875 ELISA Kit, C78335 ELISA Kit, fi03d03 ELISA Kit, zgc:55585 ELISA Kit, wu:fi03d03 ELISA Kit, zgc:111848 ELISA Kit, 6-PGDH ELISA Kit, GND1b ELISA Kit, Pgd ELISA Kit, Cc2-27 ELISA Kit, phosphogluconate dehydrogenase ELISA Kit, Phosphogluconate dehydrogenase ELISA Kit, phosphogluconate dehydrogenase L homeolog ELISA Kit, 6-phosphogluconate dehydrogenase, decarboxylating ELISA Kit, 6-phosphogluconate dehydrogenase, decarboxylating-like ELISA Kit, PGD ELISA Kit, Pgd ELISA Kit, pgd.L ELISA Kit, pgd ELISA Kit, GND1a|GND1b ELISA Kit, Thal_1580 ELISA Kit, GY4MC1_3101 ELISA Kit, Odosp_2189 ELISA Kit, Marky_1024 ELISA Kit, gnd ELISA Kit, LOC101451876 ELISA Kit

UniProt: P52209