MERTK ELISA Kit

Catalog No. ABIN6720381

Product Details MERTK ELISA Kit

Target: MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine

Analytical Method: Quantitative

Characteristics:

Synonyms: C mer, c mer proto oncogene tyrosine kinase, MER receptor tyrosine kinase, Mertk, MERTK_HUMAN, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, RP38, STK kinase, Tyrosine-protein kinase Mer

Background: Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type(immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium(RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa(RP)

Gene Name: MERTK

Production: Natural and recombinant human Mer. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: R26-A499

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Mer standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin), saliva or urine to each empty well. It is recommended that each human Mer standard solution and each sample be measured in duplicate.

ELISA Dilution: 156pg/mL-10000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for MERTK

Target: MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

Alternative Name: Mer (MERTK Products)

Synonyms: MER ELISA Kit, RP38 ELISA Kit, c-mer ELISA Kit, Eyk ELISA Kit, Mer ELISA Kit, Nyk ELISA Kit, nmf12 ELISA Kit, rdy ELISA Kit, C-EYK ELISA Kit, MERTK ELISA Kit, DKFZp469D2215 ELISA Kit, si:ch1073-268l12.2 ELISA Kit, MER proto-oncogene, tyrosine kinase ELISA Kit, c-mer proto-oncogene tyrosine kinase ELISA Kit, c-mer proto-oncogene tyrosine kinase a ELISA Kit, MERTK ELISA Kit, Mertk ELISA Kit, mertka ELISA Kit

Gene ID: 10461

NCBI Accession: NP_006334

UniProt: Q12866

Pathways: RTK Signaling