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DLL1 ELISA Kit

Rockland koa0752 DLL1 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
Rockland
Catalog No. ABIN6720373
Supplier Product No.: koa0752
  • Target See all DLL1 ELISA Kits
    DLL1 (delta-Like 1 (DLL1))
    Reactivity
    • 7
    • 5
    • 3
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0752
    Supplier
    Rockland
    Purpose
    Human DLL1 Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human DLL1. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 10 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product DLL1 ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human DLL1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), urine or human milk to each empty well. We recommend that each human DLL1 standard solution and each sample is measured in duplicate.
    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: S22-G540

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all DLL1 ELISA Kits
    DLL1 (delta-Like 1 (DLL1))
    Alternative Name
    DLL1 (DLL1 Products)
    Background

    Synonyms: Delta-like protein 1, Delta1, DLL1, DLL1_HUMAN, Drosophila Delta homolog 1, H-Delta-1

    Background: DLL1(DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

    Gene ID
    28514
    NCBI Accession
    NP_005609
    UniProt
    O00548
    Pathways
    Notch Signaling, Stem Cell Maintenance
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