APOE ELISA Kit
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- Target See all APOE ELISA Kits
- APOE (Apolipoprotein E (APOE))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 3.12 ng/mL - 200 ng/mL
- Minimum Detection Limit
- 3.12 ng/mL
- Application
- ELISA
- Purpose
- Sandwich ELISA for Quantitative Detection of Antigen
- Sample Type
- Cell Culture Supernatant, Milk, Plasma (EDTA - heparin), Saliva, Serum, Urine
- Analytical Method
- Quantitative
- Characteristics
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Synonyms: AD2, Alzheimer disease 2, Apo E, Apo-E, ApoE, APOEA, Apolipoprotein E, Apolipoprotein-E, ApolipoproteinE, Apoprotein, LDLCQ5, LPG
Background: Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimer's disease and cardiovascular disease.
Gene Name: APOE
Production: Natural human APOE. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: K19-H317
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- Discover our top product APOE ELISA Kit
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- Application Notes
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL human APOE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, milk or urine to each empty well. It is recommended that each human APOE standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 3.12 ng/mL-200 ng/mL
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Target See all APOE ELISA Kits
- APOE (Apolipoprotein E (APOE))
- Alternative Name
- APOE (APOE Products)
- Synonyms
- ad2 ELISA Kit, apoprotein ELISA Kit, im:7036787 ELISA Kit, wu:fb69a05 ELISA Kit, zgc:110064 ELISA Kit, apoe ELISA Kit, AI255918 ELISA Kit, AD2 ELISA Kit, LDLCQ5 ELISA Kit, LPG ELISA Kit, APOEA ELISA Kit, Apo-E ELISA Kit, apolipoprotein E ELISA Kit, apolipoprotein Ea ELISA Kit, apoe ELISA Kit, apoea ELISA Kit, Apoe ELISA Kit, APOE ELISA Kit
- Gene ID
- 348
- NCBI Accession
- NP_000032
- UniProt
- P02649
- Pathways
- Regulation of Cell Size, Lipid Metabolism
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