TFF3 ELISA Kit

Catalog No. ABIN6720362

Product Details TFF3 ELISA Kit

Target: TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine

Analytical Method: Quantitative

Characteristics:

Synonyms: hITF, hP1.B, Intestinal trefoil factor, ITF, mITF, OTTMUSP00000021729, Polypeptide P1.B, TFF3, TFF3_HUMAN, TFI, TREFOIL, Trefoil factor (intestinal), Trefoil factor 3

Background: Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

Gene Name: TFF3

Production: Natural and recombinant human TFF3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: E22-Y80

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TFF3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or urine to each empty well. It is recommended that each human TFF3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for TFF3

Target: TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))

Alternative Name: TFF3 (TFF3 Products)

Synonyms: TFF3 ELISA Kit, ITF ELISA Kit, P1B ELISA Kit, TFI ELISA Kit, mITF ELISA Kit, trefoil factor 3 ELISA Kit, trefoil factor 3, intestinal ELISA Kit, TFF3 ELISA Kit, Tff3 ELISA Kit

Gene ID: 7033

NCBI Accession: NP_003217

UniProt: Q07654