Angiopoietin 2 ELISA Kit

Catalog No. ABIN6720360

Product Details Angiopoietin 2 ELISA Kit

Target: Angiopoietin 2 (ANGPT2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: AGPT2, ANG 2, ANG-2, ANG2, Angiopoietin 2a, Angiopoietin 2B, Angiopoietin-2, Angiopoietin2, ANGP2_HUMAN, ANGPT 2, Angpt2, Tie2 ligand

Background: Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60 % homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Gene Name: ANGPT2

Production: Natural and recombinant human Angiopoietin-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Y19-F496

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. It is recommended that each human Angiopoietin-2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: Angiopoietin-2 (ANGPT2 Products)

Synonyms: AGPT2 ELISA Kit, ANG2 ELISA Kit, Agpt2 ELISA Kit, Ang-2 ELISA Kit, Ang2 ELISA Kit, ang2 ELISA Kit, angpt2 ELISA Kit, ANGIOPOIETIN-2 ELISA Kit, ANG-2 ELISA Kit, ANGPT2 ELISA Kit, Angrp ELISA Kit, Rnase5b ELISA Kit, ANG3 ELISA Kit, C11orf2 ELISA Kit, C11orf3 ELISA Kit, FFR ELISA Kit, angiopoietin 2 ELISA Kit, angiopoietin 2b ELISA Kit, angiogenin, ribonuclease A family, member 2 ELISA Kit, VPS51, GARP complex subunit ELISA Kit, ANGPT2 ELISA Kit, Angpt2 ELISA Kit, angpt2b ELISA Kit, Ang2 ELISA Kit, VPS51 ELISA Kit

Gene ID: 285

NCBI Accession: NP_001112359

UniProt: O15123

Pathways: RTK Signaling