Kallikrein 5 ELISA Kit

Catalog No. ABIN6720358

Product Details Kallikrein 5 ELISA Kit

Target: Kallikrein 5 (KLK5)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Kallikrein 5, Kallikrein-like protein 2, kallikrein-related peptidase 5, KLK L2, SCTE, Stratum corneum tryptic enzyme

Background: Kallikrein gene 5(KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

Gene Name: KLK5

Production: Natural and recombinant human KLK5. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: V23-S293

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human KLK5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), human milk or saliva to each empty well. We recommend that each human KLK5 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 78pg/mL-5000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Kallikrein 5

Target: Kallikrein 5 (KLK5)

Alternative Name: KLK5 (KLK5 Products)

Synonyms: KLK-L2 ELISA Kit, KLKL2 ELISA Kit, SCTE ELISA Kit, 1110030O19Rik ELISA Kit, KAL ELISA Kit, KALA ELISA Kit, Klk1 ELISA Kit, Klk5 ELISA Kit, RATKALA ELISA Kit, kallikrein related peptidase 5 ELISA Kit, kallikrein related-peptidase 5 ELISA Kit, kallikrein 5-like ELISA Kit, KLK5 ELISA Kit, Klk5 ELISA Kit, Klk5l ELISA Kit

Gene ID: 25818

NCBI Accession: NP_001070959

UniProt: Q9Y337

Pathways: Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling