IL18BP ELISA Kit

Catalog No. ABIN6720356

Supplier Product No.: koa0758

Product Details IL18BP ELISA Kit

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.7 pg/mL - 6000 pg/mL

Minimum Detection Limit: 93.7 pg/mL

Application: ELISA

Supplier Product No.: koa0758

Supplier: Rockland

Purpose: Human IL-18BP Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human IL-18BP. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m human IL-18BP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human IL-18BP standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: T31-G194

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IL18BP

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Alternative Name: IL-18BP (IL18BP Products)

Background:

Synonyms: I18BP_HUMAN, IL-18BP, IL18BP, Interleukin-18-binding protein, Tadekinig-alfa

Background: Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.

Gene ID: 10068

NCBI Accession: NP_001034748

UniProt: O95998