MIA ELISA Kit

Catalog No. ABIN6720344

Product Details MIA ELISA Kit

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD RAP, melanoma inhibitory activity, Melanoma inhibitory activity protein, Melanoma-derived growth regulatory protein, Mia, MIA_HUMAN

Background: Melanoma Inhibiting Activity(MIA), also known as cartilage-derived retinoic acid-sensitive protein(CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain(1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .

Gene Name: MIA

Production: Natural and recombinant human MIA. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: G25-131Q

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. It is recommended that each human MIA standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Alternative Name: MIA (MIA Products)

Synonyms: CD-RAP ELISA Kit, Mia1 ELISA Kit, Cdrap ELISA Kit, zgc:194157 ELISA Kit, mia ELISA Kit, CDRAP ELISA Kit, Fdp ELISA Kit, MIA ELISA Kit, MIAL ELISA Kit, melanoma inhibitory activity ELISA Kit, otoraplin ELISA Kit, cilia and flagella associated protein 100 ELISA Kit, MIA ELISA Kit, Mia ELISA Kit, mia ELISA Kit, Otor ELISA Kit, CFAP100 ELISA Kit

Gene ID: 8190

NCBI Accession: NP_001189482

UniProt: Q16674