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CCL5 ELISA Kit

Rockland koa0293 CCL5 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
Rockland
Catalog No. ABIN6720337
Supplier Product No.: koa0293
  • Target See all CCL5 ELISA Kits
    CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
    Reactivity
    • 14
    • 12
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Supplier Product No.
    koa0293
    Supplier
    Rockland
    Purpose
    Human Rantes Sandwich ELISA Kit for Quantitative Detection
    Brand
    AccuSignal™
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
    Analytical Method
    Quantitative
    Specificity

    Production: Natural and recombinant human Rantes. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity
    < 3 pg/mL
    Components
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product CCL5 ELISA Kit
  • Application Notes
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Rantes standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human Rantes standard solution and each sample be measured in duplicate.
    Comment

    Standard: Expression system for standard: E.coli, Immunogen sequence: S24-S91

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target See all CCL5 ELISA Kits
    CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
    Alternative Name
    Rantes (CCL5 Products)
    Background

    Synonyms: Beta chemokine RANTES, Beta chemokine RANTES precursor, C C motif chemokine 5, CCL5_HUMAN, Chemokine ligand 5, Chemokine CC Motif Ligand 5, EoCP, Eosinophil chemotactic cytokine, RANTES, Regulated upon activation normally T expressed and presumably secreted, SCYA5, SIS delta, SIS-delta, SISd, Small inducible cytokine A5, Small inducible cytokine subfamily A member 5, T cell specific protein p288, T cell specific protein RANTES, T-cell-specific protein RANTES, TCP 228, TCP228

    Background: Rantes(regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

    Gene ID
    6352
    NCBI Accession
    NP_002976
    UniProt
    D0EI67
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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